Turk Endod J. 2023; 8(2): 43-48 | DOI: 10.14744/TEJ.2023.85570

Do variations in the concentrations of EDTA and citric acid affect the release of different growth factors from the dentin matrix?

Dilek Hançerlioğulları, Ali Türkyılmaz, Ali Erdemir
Department of Endodontics, Kırıkkale University, Kırıkkale, Türkiye

Purpose: To investigate the release levels of transforming growth factor beta-1 (TGF-β1), bone morphogenetic protein-7 (BMP-7), insulin-like growth factor-1 (IGF-1), and vascular endothelial growth factor-A (VEGF-A) from the dentin matrix using 10% and 17% ethylenediaminetetraacetic acid (EDTA) and 10% and 20% citric acid during regenerative endodontic procedures.
Methods: Fifty mandibular single-rooted premolar teeth were used. 8-mm cylindrical roots were irrigated with 1.5% sodium hypochlorite. Root segments were divided into groups by 20 mL of final irrigants: saline, 10% or 17% EDTA, and 10% or 20% citric acid. The samples were placed into culture medium and kept in an incubator at 37°C for 24 h. The supernatants were collected to measure the levels of growth factors with an enzyme-linked immunosorbent assay. The data were evaluated using a one-way analysis of variance.
Results: EDTA or citric acid increased the release levels of all growth factors compared to the control group. The greatest amount of TGF-β1 release was measured with 17% EDTA; however, it was not significantly different from 10% citric acid. The lowest amount of BMP-7 released was with 10% citric acid, which was significantly different from the other groups.
Conclusion: The 17% EDTA and 10% citric acid solutions had a similar effect on the release of growth factors from the dentin matrix.

Keywords: Citric acid, ethylenediaminetetraacetic acid, growth factors, regenerative endodontics.

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