The effect of EDTA, phytic acid, and glycolic acid on dentinal tubule penetration of a bioceramic-based paste: A confocal study

Purpose: In this study, the effects of ethylene diamine tetraacetic acid (EDTA), phytic acid (IP6), and glycolic acid (GA) on the dentin tubule penetration of bioceramic-based sealers were evaluated by confocal laser scanning microscopy (CLSM).
Methods: A total of 60 human mandibular premolar teeth were included. The teeth were divided into four groups (n = 15): Group 1: 5.25% NaOCl + 17% EDTA, Group 2: 5.25% NaOCl + 1% IP6, Group 3: 5.25% NaOCl + 10% GA, and Group 4: 5.25% NaOCl + distilled water (DW). The canals were filled with Bioserra canal paste mixed with 0.1% Rhodamine B.
Results: The penetration depth and percentage of the bioceramic-based paste were significantly higher in all groups than in the control group. However, this difference was not significant for the penetration area.
Conclusion: GA and IP6 are similar to EDTA in terms of their effects on bioceramic paste penetration.