Careful evaluation of the internal anatomy of a root canal is critical for successful endodontic treatment. An additional root or missing canal can lead to treatment failures and poor prognosis. The two palatal canals in the maxillary second molar tooth are rare, and its incidence reported in the literature is less than 2%. The unique anatomy of the maxillary second molar teeth is complex to treat due to its posterior location. Superimposition of the anatomical structures on the radiographs of this region may result in a second palatal root canal undiagnosed. The current case report presents non-surgical re-treatment of maxillary second molar with two palatal roots. CBCT image confirmed the presence of nontreated palatal root. The extra palatal root of the tooth had been treated, and the patient’s symptoms resolved.Keywords: Cone beam computed tomography, maxillary second molar, second palatal root.